A simple, specific and sensitive reverse phase high performance liquid chromatographic (RP-HPLC) method was developed and validated for simultaneous determination of linagliptin and empagliflozine in tablet dosage forms. Chromatography was performed through kromosil (250 x 4.6 mm, 5m) and isocratic elution. Mobile phase containing buffer and acetonitrile in the ratio of 70:30 was pumped through column at a flow rate of 1 ml/min. Buffer used in this method was 0.1% OPA buffer with pH 4.8 adjusted by triethylamine maintained with a temperature of 30°C. Optimized wavelength for linagliptin and empagliflozine was 286 nm. Retention time of linagliptin and empagliflozine were found to be 1.920 min and 3.699 min respectively. %RSD of the linagliptin and empagliflozine were 1.0 and 0.94 respectively. All the validation parameters were in the acceptable range. Simultaneously this method applied to determine the degradation products of linagliptin and empagliflozine. The detection wavelength of 286 nm was chosen in order to achieve high sensitivity for quantitative determination of these drugs in solid dosage form. Method can be successfully employed for simultaneous estimation of these drugs in commercial products.
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